Constitutive  Aspm knock-out alleles.
Aspm knockouts generated using CRISPR/Cas9 techniques in mouse embryos.

Inducible overexpression of Aurora A.
The Aurka cDNA was inserted downstream of tet-responsive ColA1 sequences using KH2 mouse embryonic stem (ES) cells following the strategy reported by Beard et al. 2006, Genesis 44, 23–28 [PubMed]. Induction is achieved after the activation of the reverse tetracycline transactivator (rtTA; expressed in a different allele in the Rosa26 locus) with the tetracycline derivative doxycycline

Constitutive and conditional Aurkb knock-out alleles.
Complete ablation of the Aurora B-encoding gene results in lethality both in embryos and adult mice.

Available at EMMA: EM:06109

Inducible overexpression of the endogenous murine Aurora B-encoding gene.
Tetracycline-responsive elements were inserted in the endogenous Aurkb gene either in the presence [Aurkb(loxtet)] or absence [Aurkb(tet)] of loxP sequences. Overexpression of Aurora B results in genomic instability and increased tumor susceptibility.

Germline knockout alleles of Brk1.
Brk1 (also known as Hspc300) encodes the Brick1 protein involved in actin dynamics. Brk1 ablation results in early embryonic lethality.

Inducible overexpression of Cyclin Y.
The Ccny cDNA was inserted downstream of tet-responsive ColA1 sequences using KH2 mouse embryonic stem (ES) cells following the strategy reported by Beard et al. 2006, Genesis 44, 23–28 [PubMed]. Induction is achieved after the activation of the reverse tetracycline transactivator (rtTA; expressed in a different allele in the Rosa26 locus) with the tetracycline derivative doxycycline

Germline and conditional knockout alleles of Cdc14a.

Germline and conditional knockout alleles of Cdc14b.
Cdc14b-deficient mice are viable and display increased tumor susceptibility.

Constitutive and conditional Cdc20 knock-out alleles.
Complete ablation of Cdc20 results in lethality both in embryos and adult mice.

Available at EMMA: EM:06117

Knockin allele of Cdk4 carrying the R24C mutation.
The Cdk4 R24C mutation was found in melanoma patients. Cdk4 R24C is insensitive to INK4 family of inhibitors resulting in increased susceptibility to tumor development. It also cooperates with the Cdk6 R31C mutation.

Constitutive Cdk5rap2 knock-out alleles.
Cdk5rap2 knockouts generated using CRISPR/Cas9 techniques in mouse embryos.

Germline conditional knockout allele of Cdk6.
Cdk6-deficient mice are viable and display minor defects in red blood counts. This allele results in embryonic lethality in mid-gestation when combined with a Cdk4-null allele.

Knockin allele of Cdk6 carrying the R31C mutation.
Cdk6 R31C protein is insensitive to INK4 family of inhibitors resulting in increased susceptibility to tumor development. It also cooperates with the Cdk4 R24C mutation.

Constitutive and conditional Cdk14 knock-out alleles.
Germline and conditional knockout alleles of the Cdk14 gene.

Constitutive Cep135 knock-out alleles.
Cep135 knockouts generated using CRISPR/Cas9 techniques in mouse embryos.

Constitutive and conditional Fzr1 (Cdh1) knock-out alleles.
Germline and conditional knockout alleles of the Fzr1 gene, encoding the Anaphase-promoting complex (APC/C)-cofactor Cdh1. Complete ablation of the Cdh1-encoding gene results in embryonic lethality due to placental defects.

Available at EMMA: EM:06108

Constitutive and conditional Gsg2 (Haspin) knock-out alleles.
Germline and conditional knockout alleles of the Gsg2 gene, encoding the kinase Haspin.

Constitutive Hec1 (also kwon as Ndc80) gene-trap allele.
In progress

Constitutive Mad1 gene-trap allele.
In progress

Constitutive and conditional Mastl knock-out alleles.
Complete ablation of Mastl results in embryonic lethality.

Mastl E166D knockin allele carrying the mutation found in thrombocytopenia patients.
Mastl E166D express normal levels of the mutant Mastl protein. These mice develop mild thrombocytopenia due to defects in platelet activation and alterations in the signaling pathways controlling actin dynamics.

Mastl alleles expressing a truncated form of Mastl.
Mastl alleles carrying specific insertion or deletions in the linker region and expressing short forms of the kinase. These alleles were generated using direct injection of CRISPR/Cas9 reagents in mouse embryos.

Numa gene-trap allele.
In progress

Constitutive and conditional Plk1 knock-out alleles.
Complete ablation of the Plk1-encoding gene results in lethality both in embryos (Wachowicz et al., Bioessays 2016) and adult mice (de Cárcer et al., Nat. Med. 2017), as well as specific defects in megakaryocytes (Trakala et al. Bood 2015) and smooth muscle cells (de Cárcer et al., Nat Med. 2017).

Inducible overexpression of Plk1.
A FLAG-tagged human Plk1 gene was inserted downstream of tet-responsive ColA1 sequences using KH2 mouse embryonic stem (ES) cells following the strategy reported by Beard et al. 2006, Genesis 44, 23–28 [PubMed]. Induction is achieved after the activation of the reverse tetracycline transactivator (rtTA; expressed in a different allele in the Rosa26 locus) with the tetracycline derivative doxycycline.

Constitutive and conditional Ppp2r2a (encoding B55alpha) alleles.
In progress

Constitutive Ppp2r2b (encoding B55beta) allele.
In progress

Constitutive Ppp2r2c (encoding B55gamma) allele.
In progress

Constitutive Ppp2r2d (encoding B55delta) allele.
In progress

Constitutive Rec8 knockout allele.
Cep135 knockouts generated using CRISPR/Cas9 techniques in mouse embryos.

Constitutive Spo11 knockout allele.
Spo11 knockouts generated using CRISPR/Cas9 techniques in mouse embryos.

Constitutive Stag3 knockout allele.
Stag3 knockouts generated using CRISPR/Cas9 techniques in mouse embryos.

Constitutive and conditional Tpx2 gene-trap alleles.
Germline knockout of Tpx2 results in embryonic lethality. Conditional ablation of the gene (using the FLIP gene-trap system) results in chromosomal instability and increased susceptibility to tumor development.

Constitutive Trip13 knockout allele.
Trip13 knockouts generated using CRISPR/Cas9 techniques in mouse embryos.

Constitutive Wdr62 knockout allele.
Wdr62 knockouts generated using CRISPR/Cas9 techniques in mouse embryos.

Knock-in of miR-203, inducible by DOXYCYCLINE treatment, through the Rosa 26_rtTA allele. Reverse tetracycline transactivator (rtTA)/tetracycline-responsive element (tet-O)-driven transgenes. To ensure reliable rtTA expression in a broad range of cell types, the rtTA transgene has been targeted into the ROSA26 locus. Transgen PyMT, constitutively active (not inducible), under the promoter of MMTV, only expressed in breast. Tumoral invasive lessions are observed in breast at 15 -16 weeks of age. Long latency model of breast cancer

Knock-out of miR-203 and Knock-in for OSKM (Oct4-Sox2-Klf4-Myc). The OSKM inducible strain was kindly provided by Manuel Serrano´s lab in 2014. We crossed the OSKM strain with our BED strain, knock out for miR-203. OSKM transgen is inducible by DOXYCYCLINE treatment, using Rosa 26_rtTA allele. Reverse tetracycline transactivator (rtTA)/tetracycline-responsive element (tet-O)-driven transgenes. To ensure reliable rtTA expression in a broad range of cell types, the rtTA transgene has been targeted into the ROSA26 locus.