Resources

Genotyping Protocols

ColA_miR-203; Rosa 26_ rtTA; OSKM

Knock-in of miR-203 and OSKM (Oct4-Sox2-Klf4-Myc). The OSKM inducible strain was kindly provided by Manuel Serrano´s lab in 2014. We crossed the OSKM strain with our BGC strain, knock in for miR-203. Both miR-203 and OSKM transgen are inducible by DOXYCYCLINE treatment. through the Rosa 26_rtTA allele. Reverse tetracycline transactivator (rtTA)/tetracycline-responsive element (tet-O)-driven transgene. To ensure reliable rtTA expression in a broad range of cell types, the rtTA transgene has been targeted into the ROSA26 locus.

Unpublished

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ColA_miR-203; Rosa 26_ rtTA; MMTV_PyMT

Knock-in of miR-203, inducible by DOXYCYCLINE treatment, through the Rosa 26_rtTA allele. Reverse tetracycline transactivator (rtTA)/tetracycline-responsive element (tet-O)-driven transgenes. To ensure reliable rtTA expression in a broad range of cell types, the rtTA transgene has been targeted into the ROSA26 locus. Transgen PyMT, constitutively active (not inducible), under the promoter of MMTV, only expressed in breast. Tumoral invasive lessions are observed in breast at 15 -16 weeks of age. Long latency model of breast cancer

Unpublished

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ColA_miR-203; Rosa 26_ rtTA

Knock-in of miR-203, inducible by DOXYCYCLINE treatment, through the Rosa 26_rtTA allele.Reverse tetracycline transactivator (rtTA)/tetracycline-responsive element (tet-O)-driven transgenes. To ensure reliable rtTA expression in a broad range of cell types, the rtTA transgene has been targeted into the ROSA26 locus.

Unpublished

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miR-203 KO

Knock out for microRNA 203

Unpublished

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miR-203 KO; Rosa 26_ rtTA; OSKM

Knock-out of miR-203 and Knock-in for OSKM (Oct4-Sox2-Klf4-Myc). The OSKM inducible strain was kindly provided by Manuel Serrano´s lab in 2014. We crossed the OSKM strain with our BED strain, knock out for miR-203. OSKM transgen is inducible by DOXYCYCLINE treatment, using Rosa 26_rtTA allele. Reverse tetracycline transactivator (rtTA)/tetracycline-responsive element (tet-O)-driven transgenes. To ensure reliable rtTA expression in a broad range of cell types, the rtTA transgene has been targeted into the ROSA26 locus.

Unpublished

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Mastl_KO

Constitutive Mastl knock-out (whole body). Embryonically lethal.

Álvarez-Fernández, et al. (2013) Greatwall is essential to prevent mitotic collapse after nuclear envelop breakdown in mammals. Proc. Natl. Acad. Sci. USA. 110, 17374-17379. [PMID: 24101512] [Full text]

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CDC20_lox, Pol2a_IRES-CreERT2

Knock out for CDC20, inducible by tamoxifen treatment through Pol2a_IRES-CreERT2

Manchado, E., et al. (2010) Targeting mitotic exit leads to tumor regression in vivo: modulation by Cdk1, Mastl, and the PP2A/B55a,d phosphatase. Cancer Cell 18, 641-654. [PMID: 21156286]

[Full text]

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 Protocols